Basics of Genetic Engineering

Laboratory R&D Cell and Gene Therapy @Biotech Training Facility

Genetic engineering is a powerful tool used to study and manipulate genes in living organisms. This hands-on course provides an in-depth understanding of genetic engineering methods, especially molecular cloning, including DNA isolation, PCR techniques, and genome editing technologies.

Location

Biotech Training Facility

Duration

3 days

Course fee

€ 2,550.00 (excl. VAT)

This course is only available for group registrations of 6 trainees or more. Please contact us at info@biotechtrainingfacility.nl to arrange a date.

Short description

Genes in our genome make up the complete blueprint for our cells to work properly. Understanding the structural composition and functions of genes is crucial to find out how genes work and how they cause diseases.

This course covers fundamental research methods for genetic studies. Participants will learn about the structures and functions of genes and genomes, as well as gain hands-on experience in isolating DNA, designing primers, performing PCR, and molecular cloning.

In the biopharmaceutical industry, molecular cloning plays a crucial role in the development of therapeutic proteins, monoclonal antibodies and vaccines. By cloning and expressing specific genes, large quantities of biologically active compounds can be produced for medical treatments, drug discovery and industrial applications.

Participants will gain both theoretical knowledge and practical laboratory experience.

Objectives

Acquire a thorough understanding of how genes work.
Gain proficiency in performing molecular cloning techniques.
Assess the factors affecting the results and to be able to troubleshoot.

Target group

This course is suitable for researchers and laboratory professionals working in biotechnology and related fields.

Program

Theory: Introduction to genes and genomes.
Structure and functions of genes and genomes. Comparison of genomes in different organisms. Special features of genes and genomes.
Theory: Methods in genomic research.
History of genomic research. Fundamentals of methods used in genomic research. Up-to-date research processes.
Practice: Genomic DNA isolation.
Genomic DNA isolation from human cheek epithelial cells.
Theory: Primer design and PCR methods
Designing primers with online tools. Important features of primer design. Setting up a PCR application. Tips about optimization and good PCR results. Troubleshooting methods. Different types of PCRs.
Practice: PCR application
Practical application of a PCR for a desired gene in genomes.
Practice: Agarose gel electrophoresis
Visualization of PCR results with agarose gel electrophoresis. Preparing the agarose gel. Loading and running the samples.

Practice: Plasmid digestion and DNA ligation
Digestion of a plasmid with restriction endonucleases. Insertion of genetic material into plasmids with T4 ligase. Different type of vectors for transformation and their applications in genetic engineering.
Practice: Transformation
Plasmid transformation to competent E. coli cells with heat-shock.
Practice: E. coli culture
Culturing transformed E. coli cells to the selective media.

Practice: Colony analysis
Analysis of colonies on selective media. Assessments of results.
Practice: Colony PCR
PCR application for the inserted genetic material directly from colonies. Advantages of colony PCR.
Practice: Plasmid isolation
Plasmid DNA isolation from colonies. Important features of plasmid isolation.
Practice: Agarose gel electrophoresis
Visualization of results with agarose gel electrophoresis. Assessments of results.

Interested in signing up a group of people for this course?

Please contact our Business Development team: info@biotechtrainingfacility.nl +31 (0)88 2830100

email to BTF